Molecular Mechanisms of Cell Division
Genetics, Cell Biology, Biochemistry and Biophysics
Our group is interested in understanding the mechanism underlying the process of cell division and its regulation. We use a combination of in vitro and in vivo approaches including genetic, cellular, molecular and mathematical approaches to understand the assembly and function of the cell division apparatus. We use the fission yeast as a simple model system to carry out most of our studies. As the process of cytokinesis and its regulation is mechanistically conserved, our findings should be applicable to understanding how cell division is controlled in human cells. Failure to complete cytokinesis results in aneuploidy associated with infertility, developmental defects and genetic instability that often lead to cancer. Hence, studying cytokinesis has important implications for cell proliferation and differentiation in normal development and also in disease conditions.
Mishra, M., Kashiwazaki, J., Takagi, T., Srinivasan, R., Huang, Y., Balasubramanian, M.K., and Mabuchi, I. (2013). In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics. Nat Cell Biol 15, 853-859.
Mishra, M., Huang Y, Srivastava P, Srinivasan R, Sevugan M, Shlomovitz R, Gov N, Rao M, Balasubramanian M.(2012). Cylindrical cellular geometry ensures fidelity of division site placement in fission yeast. J Cell Sci .125, 3850-3857.
Mishra, M., Oliferenko S. (2008). Cytokinesis: catch and drag. Curr Biol 18:R247-R250.
Srinivasan R, Mishra, M., Wu L, Yin Z, Balasubramanian MK (2008) The bacterial cell division protein FtsZ assembles into cytoplasmic rings in fission yeast. Genes Dev 22(13):1741-1746.
Mishra, M., Karagiannis, J. et al.(2005) The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast. Current Biology: CB 15, 1376-1383.
Mishra, M., Karagiannis, J., Trautmann, S., Wang, H., McCollum, D., and Balasubramanian, M.K. (2004). The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe. J Cell Sci 117, 3897-3910.
Motegi F, Mishra, M., Balasubramanian MK, Mabuchi I. (2004). Myosin- II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast. J Cell Biol 165:685-695.