Genetic mechanisms and signaling pathways in the developing brain
Cerebral cortex and the amygdala are the two most complex brain structures. The cortex is responsible for higher brain functions- perception, language, learning, memory; the amygdala is the "emotion center" of the brain. The broad interests of our group are in the genetic and epigenetic mechanisms that control the formation of these highly specialized structures. We use "knockout" and transgenic approaches, in-utero and tissue culture perturbation techniques, ChIP-seq and Mass spectrometry, neurocircuitry tracing and CLARITY-based approaches, and behavioral paradigms to pursue these questions.
Bhavana M, Khatri Z, Maheshwari U, Gupta R, , Roy B, Pradhan S, Karmodiya K, Padmanabhan H, Shetty AS, Balaji CH, Kolthur U, Macklis JD, Galande S, and Tole S (2016). Lhx2 interacts with the NURD complex and epigenetically modulates cortical neuron subtype regulators Fezf2 and Sox11 Journal of Neuroscience 2836-16. PMID: 27909100 PDF Here..
Roy A, Gonzalez M, Pierani A, Meyer G,Tole S# (2014) Lhx2 regulates the development of the forebrain hem system. Cerebral Cortex 24(5):1361-72 PDF Here..
Shetty AS, Godbole G, Maheshawari U, Padmanabhan H, Chaudhary R, Muralidharan B, Hou P-S, Monuki ES, Kuo H-C, V Rema, Tole S# (2013) Lhx2 regulates a cortex-specific mechanism for barrel formation PNAS doi:10.1073/pnas.1311158110 PDF Here..
Huilgol D, Udin, S, Shimogori T, Saha B, Roy A, Aizawa S., Hevner RF, Meyer G., Ohshima T, Pleasure SJ, Zhao Y, Tole S# (2013)Dual origins of the mammalian accessory olfactory bulb revealed by an evolutionarily conserved migratory stream Nature Neuroscience 16(2):157-65 PDF Here..
Subramanian L, Sarkar A, Ashwin S, Muralidharan B, Hari P, Piper M, Monuki ES, Bach I,Gronostajski R, Richards R, andTole, S# (2011)Transcription factor Lhx2is necessary and sufficient to suppress astrogliogenesis and promote neurogenesis in the developing hippocampus.
PNAS www.pnas.org/cgi/doi/10.1073/pnas.1101109108 PDF Here..
Mangale VS, Hirokawa KE, Satyaki PRV, GokulchandranN, ChikbireS, Subramanian L, Shetty AS, Martynoga B, Paul J, Mai MV, Li Y, Flanagan LA, Tole S#, and Monuki ES # (2008) Lhx2 selector activity specifies cortical identity and suppresses hippocampal organizer fate Science 319:304-309# co-corresponding authors PDF Here..
Remedios, R., Huilgol, D., Saha, B., Hari, P., Bhatnagar, L., Kowalczyk T., Hevner R. F., Suda Y., Aizawa S., Ohshima, T., Stoykova, A., Tole, S#. (2007) A novel stream of amygdaloid cells from the caudal telencephalon reveals a developmental link between the amygdala and the neocortex
Nature Neuroscience 10 (9): 1141–1150 PDF Here..
Saha, B. Hari P., Huilgol, D. and Tole, S#. (2007) Dual role for LIM-HD gene Lhx2 in the formation of the lateral olfactory tract (LOT) Journal of Neuroscience 27: 2290-2297 PDF Here..
Huilgol D and Tole S# (2016) Cell migrations in the developing rodent olfactory system.
Cellular and Molecular Life Sciences 73(13), 2467-2490 PDF Here..
Subramanian L, and Tole S#.(2009) Mechanisms underlying the specification, positional regulation and function of the cortical hem. Cerebral Cortex 19 (Suppl 1):i90-5. PDF Here..
Tole S. and Vale, R. (2010). Young leaders for Biology in India. Science 329: 1441. (Editorial)
Tole S. and Hebert J. Telencephalon Patterning. “Cellular Migration and Formation of Neuronal Connections: Comprehensive Developmental Neuroscience” (2013) John Rubenstein and PaskoRakic (Eds). Academic Press. ISBN: 978-0-12-397265-1