ÿþ<html> <head> <meta http-equiv=Content-Type content="text/html; charset=unicode"> <meta name=Generator content="Microsoft Word 12 (filtered)"> <title>Movies from the Mallik Lab, TIFR-Mumbai</title> <style> <!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4;} @font-face {font-family:Tahoma; panose-1:2 11 6 4 3 5 4 4 2 4;} @font-face {font-family:"Lucida Sans Unicode"; panose-1:2 11 6 2 3 5 4 2 2 4;} @font-face {font-family:Verdana; panose-1:2 11 6 4 3 5 4 4 2 4;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {margin:0cm; margin-bottom:.0001pt; font-size:12.0pt; font-family:"Times New Roman","serif"; color:windowtext;} h1 {mso-style-link:"Heading 1 Char"; margin-right:0cm; margin-left:0cm; font-size:18.0pt; font-family:"Verdana","sans-serif"; color:silver; font-weight:bold;} h2 {mso-style-link:"Heading 2 Char"; margin-right:0cm; margin-left:0cm; font-size:10.5pt; font-family:"Verdana","sans-serif"; color:silver; font-weight:bold;} a:link, span.MsoHyperlink {color:silver; text-decoration:none none;} a:visited, span.MsoHyperlinkFollowed {color:purple; text-decoration:none none;} p {margin-right:0cm; margin-left:0cm; font-size:7.0pt; font-family:"Verdana","sans-serif"; color:silver;} p.MsoAcetate, li.MsoAcetate, div.MsoAcetate {mso-style-link:"Balloon Text Char"; margin:0cm; margin-bottom:.0001pt; font-size:8.0pt; font-family:"Tahoma","sans-serif"; color:windowtext;} span.Heading1Char {mso-style-name:"Heading 1 Char"; mso-style-link:"Heading 1"; font-family:"Cambria","serif"; color:#365F91; font-weight:bold;} span.Heading2Char {mso-style-name:"Heading 2 Char"; mso-style-link:"Heading 2"; font-family:"Cambria","serif"; color:#4F81BD; font-weight:bold;} span.BalloonTextChar {mso-style-name:"Balloon Text Char"; mso-style-link:"Balloon Text"; font-family:"Tahoma","sans-serif";} .MsoChpDefault {font-size:10.0pt;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt;} div.Section1 {page:Section1;} --> </style> </head> <body bgcolor=black lang=EN-US link=silver vlink=purple> <div class=Section1> <h1 align=center style='margin:0cm;margin-bottom:.0001pt;text-align:center'><i><span style='font-size:12.0pt;color:#FF9900'>&nbsp;</span></i></h1> <h2 align=center style='margin-top:0cm;margin-right:0cm;margin-bottom:6.0pt; margin-left:0cm;text-align:center'><span style='font-size:14.0pt;font-family: "Lucida Sans Unicode","sans-serif";color:#CC99FF;font-weight:normal'>Mallik Lab. Tata Institute of Fundamental Research, Mumbai, India</span></h2> <p class=MsoNormal align=center style='text-align:center;text-indent:18.0pt'><i><span style='font-size:10.0pt;font-family:"Lucida Sans Unicode","sans-serif"; color:#CC99FF'><a href="http://www.tifr.res.in/%7Eroop/"><span style='color:#CC99FF'>Home</span></a> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.tifr.res.in/%7Eroop/PI.htm"><span style='color:#CC99FF'>Principal Investigator</span></a>&nbsp;&nbsp;&nbsp;&nbsp; <a href="http://www.tifr.res.in/%7Eroop/Publications.htm"><span style='color:#CC99FF'>Publications</span></a>&nbsp;&nbsp;&nbsp;&nbsp; <a href="http://www.tifr.res.in/%7Eroop/Funding.htm"><span style='color:#CC99FF'>Funding</span></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <a href="http://www.tifr.res.in/%7Eroop/Contact.html"><span style='color:#CC99FF'>Contact</span></a> &nbsp;&nbsp;<a href="http://www.tifr.res.in/%7Eroop/Vacancies.html"><span style='color:#CC99FF'>Vacancy</span></a>&nbsp;&nbsp;&nbsp; <u><a href="http://www.tifr.res.in/%7Eroop/LabMembers"><span style='color:#CC99FF'>LabMembers</span></a></u>&nbsp;&nbsp; &nbsp;&nbsp;<a href="http://www.tifr.res.in/%7Eroop/Images/"><span style='color:#CC99FF'>Images</span></a></span></i><i><span style='font-size: 10.0pt;font-family:"Lucida Sans Unicode","sans-serif";color:silver'>&nbsp;&nbsp; &nbsp;&nbsp;</span></i><i><span style='font-family:"Lucida Sans Unicode","sans-serif"; color:#FFCC00'><a href="http://www.tifr.res.in/%7Eroop/Movies/"><span style='color:#FFCC00'>M</span><span style='font-size:9.0pt;color:#FFCC00'>OVIES</span></a> </span></i><i><span style='font-size:10.0pt;font-family:"Lucida Sans Unicode","sans-serif"; color:silver'>&nbsp;&nbsp;&nbsp;</span></i><i><span style='font-size:10.0pt; font-family:"Lucida Sans Unicode","sans-serif";color:#CC99FF'><a href="http://www.tifr.res.in/%7Eroop/NaturesNanotech.htm"><span style='color:#CC99FF'>Molecular Motors ??</span></a>&nbsp;&nbsp;&nbsp;&nbsp; <a href="http://www.tifr.res.in/%7Eroop/Links.html"><span style='color:#CC99FF'>Links</span></a></span></i><i><span style='font-size:10.0pt;font-family:"Lucida Sans Unicode","sans-serif"; color:silver'>&nbsp; </span></i></p> <h1 style='margin:0cm;margin-bottom:.0001pt'><i><span style='font-size:10.0pt; color:#FF9900;font-weight:normal'>&nbsp;</span></i></h1> <h1 style='margin:0cm;margin-bottom:.0001pt'><i><span style='font-size:10.0pt; color:#FF9900;font-weight:normal'>&nbsp;</span></i></h1> <h1 style='margin:0cm;margin-bottom:.0001pt'><span style='font-size:11.0pt; color:#FF9900;font-weight:normal'>All movies play in real-time</span></h1> <h1 style='margin:0cm;margin-bottom:.0001pt'><span style='font-size:10.0pt; color:#FF9900;font-weight:normal'>Copyright, R. Mallik, 2007</span></h1> <h1 style='margin:0cm;margin-bottom:.0001pt'><span style='font-size:11.0pt; color:#FF9900;font-weight:normal'>&nbsp;</span></h1> <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0 style='border-collapse:collapse;border:none'> <tr style='height:143.0pt'> <td width=479 style='width:359.6pt;border:solid #999999 1.0pt;padding:0cm 5.4pt 0cm 5.4pt; height:143.0pt'> <h1 align=center style='margin:0cm;margin-bottom:.0001pt;text-align:center'><i><span style='font-weight:normal'><img border=0 width=110 height=112 id="Picture 1" src="index_files/image002.gif" alt="Diffraction rings of Optical Trap"></span></i></h1> <p class=MsoNormal align=center style='margin-top:6.0pt;text-align:center'><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:silver'>Diffraction rings of an optical trap</span></p> <p class=MsoNormal style='margin-top:6.0pt;text-align:justify'><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:silver'>&nbsp;</span></p> </td> <td width=584 style='width:438.0pt;border:solid #999999 1.0pt;border-left: none;padding:0cm 5.4pt 0cm 5.4pt;height:143.0pt'> <p class=MsoNormal align=center style='text-align:center'><b><i><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'><img border=0 width=370 height=52 id="Picture 2" src="index_files/image003.gif" alt="Kinesin Bead assay"></span></i></b></p> <p class=MsoNormal style='margin-top:6.0pt;text-align:justify'><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:silver'>Multiple kinesin driven motility of 500 nanometer latex beads on a single microtubule. These beads move with an average velocity of 670 nanometers/second, and are driven by kinesin isolated from goat brains. The 10 second clip plays in real time. Field of view is ~30 microns horizontally. Faint outline of a single microtubule is visible</span></p> </td> </tr> <tr style='height:8.5pt'> <td width=479 style='width:359.6pt;border:solid #999999 1.0pt;border-top: none;padding:0cm 5.4pt 0cm 5.4pt;height:8.5pt'> <h1 align=center style='margin-top:0cm;margin-right:0cm;margin-bottom:6.0pt; margin-left:0cm;text-align:center'><i><span style='font-weight:normal'><img border=0 width=124 height=62 id="Picture 3" src="index_files/image004.gif" alt="Kinesin in Trap"></span></i></h1> <p class=MsoNormal align=center style='margin-top:6.0pt;text-align:center'><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:silver'>Single kinesin driven 500nm bead stalling in optical trap (watch carefully).</span></p> <h1 align=center style='margin-top:0cm;margin-right:0cm;margin-bottom:6.0pt; margin-left:0cm;text-align:center'><span style='font-size:9.0pt;font-family: "Arial","sans-serif";font-weight:normal'>A single microtubule (out of focus) is oriented along the horizontal.</span></h1> <p class=MsoNormal style='margin-top:6.0pt;text-align:justify'><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:silver'>&nbsp;</span></p> </td> <td width=584 style='width:438.0pt;border-top:none;border-left:none; border-bottom:solid #999999 1.0pt;border-right:solid #999999 1.0pt; padding:0cm 5.4pt 0cm 5.4pt;height:8.5pt'> <p class=MsoNormal align=center style='text-align:center'>&nbsp;</p> <p class=MsoNormal align=center style='text-align:center'><img border=0 width=414 height=40 id="Picture 4" src="index_files/image001.gif" alt=DictyVesicles-PolLabelledMT></p> <p class=MsoNormal style='margin-bottom:6.0pt;text-align:justify'><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:silver'>In vitro motion of endosomes isolated from <i>Dictyostelium</i> cells. A large and a smaller endosome move to cross each other on a single microtubule. We have investigated the number and force-generating properties of opposing motor-teams on these endosomes (</span><i><span style='font-size:9.0pt; font-family:"Arial","sans-serif";color:#CC99FF'><a href="http://www.tifr.res.in/~roop/Publications.htm"><span style='color:#CC99FF'>Soppina et al, PNAS 2009</span></a>)</span></i><span style='font-size:9.0pt; font-family:"Arial","sans-serif";color:silver'>. Stationary avidin coated magnetic beads (extreme right) label the minus end of the microtubule (</span><i><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:#CC99FF'><a href="http://www.tifr.res.in/~roop/Publications.htm"><span style='color:#CC99FF'>Soppina et al, Biotechniques 2009</span></a></span></i><span style='font-size:9.0pt; font-family:"Arial","sans-serif";color:silver'>) </span></p> </td> </tr> </table> <h1 style='margin:0cm;margin-bottom:.0001pt'><span style='font-size:11.0pt; color:#FF9900;font-weight:normal'>&nbsp;</span></h1> <p class=MsoNormal><b><i><span style='color:silver'>&nbsp;</span></i></b></p> <p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"; color:silver'>&nbsp;</span></p> <p class=MsoNormal><b><i><span style='color:silver'>&nbsp;</span></i></b></p> <p class=MsoNormal><b><i><span style='color:silver'>&nbsp;</span></i></b></p> <p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"; color:silver'>&nbsp;</span></p> <p class=MsoNormal><b><i><span style='font-size:10.0pt;font-family:"Arial","sans-serif"; color:silver'>&nbsp;</span></i></b></p> <p class=MsoNormal><b><i><span style='font-size:10.0pt;font-family:"Arial","sans-serif"; color:silver'>&nbsp;</span></i></b></p> <p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"; color:silver'>&nbsp;</span></p> <p class=MsoNormal><b><i><span style='font-family:"Verdana","sans-serif"; color:silver'>&nbsp;</span></i></b></p> <p class=MsoNormal><b><i><span style='color:silver'>&nbsp;</span></i></b></p> <p class=MsoNormal><b><i><span style='color:white'>&nbsp;</span></i></b></p> <p>&nbsp;</p> <p class=MsoNormal>&nbsp;</p> </div> </body> </html>