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V Soppina, A K Rai, A J Ramaiya, P Barak
and R Mallik
Tug-of-war
between Dissimilar Teams of Microtubule Motors Regulates Transport and Fission
of Endosomes
Proceedings of the National
We investigate the role of motor
proteins in endosome transport and fission through quantitative biophysical
measurements. Opposing teams of
Kinesin and Dynein motors are shown to engage in a molecular tug-of-war on
single endosomes. Surprisingly, many (4 to 8) weak and detachment-prone dyneins
are in tug-of-war against a single strong and tenacious kinesin. We show how
this clever choice of opposing motor-teams allows efficient minus-end transport
(required for endosome maturation)
concurrent with controlled fission of endosomes (required for receptor recycling). This appears to be the first demonstration
that the two major microtubule motors (Kinesin and Dynein) function differently
at the molecular level inside cells, and of how this difference may regulate an
important cellular process, namely endosome biogenesis.
V Soppina, A K Rai and R Mallik
Simple
non-fluorescent polarity labeling of Microtubules for Molecular Motor assays
Biotechniques 46, 297 (2009) PDF Abstract
A new method to identify the
polarity (minus and plus) of in vitro assembled microtubules. We reconstitute
motion of endosomes extracted from cells on the labelled microtubules. The opposing
activity of dynein (moves towards minus-end of MT) and kinesin (moves towards
plus-end) on individual endosomes can be resolved with high spatial and
temporal resolution. This will help understand how these two opposing motors
compete while moving their cargo (e.g. the endosome) on a microtubule inside
cells.
D. Petrov, R. Mallik, G.T. Shubeita, M. Vershinin, S.P. Gross,
and C. Yu
Studying Molecular
Motor-based Cargo Transport: What is Real, and What is Noise ?
Biophysical Journal 92,
2953 (2007) PDF
Abstract
R. Mallik, D. Petrov, S. A. Lex, S.J. King and
S.P. Gross
Building complexity: an in vitro study of
cytoplasmic dynein with in vivo implications.
Current Biology 15, 2075 (2005) PDF Abstract
Commentary on this paper, published in Current
Biology PDF Abstract
M.P.
Singh, R. Mallik, S.P. Gross and
C. Yu
Proceedings
of the National
R. Mallik and S.P. Gross
Molecular
motors: Strategies to get along. (Review)
Current
Biology 14, R971 (2004) PDF Abstract
R.
Mallik, B.C. Carter,
Cytoplasmic Dynein functions as a gear
in response to load.
Nature
427, 649-652 (2004) PDF
Abstract
Commentary on this paper, published in Current
Biology PDF Abstract
R. Mallik, J.B. Udgaonkar and G. Krishnamoorthy
Kinetics
of proton transfer in a green fluorescent protein: A laser-induced pH jump
study.
Proceedings
of Indian
CONDENSED MATTER PHYSICS
E.V. Sampathkumaran, R. Mallik, P.L.
Paulose & S. Majumdar (2000).
Silence of magnetic layers to magnetoresistive
process and eletronic separation at low temperatures in (La,Sm)Mn2Ge2 Phys. Lett. 71, 123.
E.V. Sampathkumaran, Subham Majumdar, R.
Mallik, R.Vijayraghavan, H. Wada and M. Shiga (2000).
Unusually large
negative magnetoresistance around 200 K in the alloys Gd1-xLax
Mn2Ge2 J. Phys.: Cond. Matter 12, L399
S. R. Saha, H. Sugawara, T. D. Matsuda, H.
Sato, R. Mallik and E. V. Sampathkumaran (1999).
Magnetic anisotropy, first-order-like
metamagnetic transitions and large negative magnetoresistance in the single
crystal of Gd2PdSi3. Phys. Rev. B 60, 12162.
S. Majumdar, R. Mallik, E.V.
Sampathkumaran, K. Rupprecht and G. Wortmann (1999)
Magnetic behaviour of
Eu2CuSi3 : Large negative magnetoresistance above Curie
temperature Phys. Rev. B 60, 6770.
Subham Majumdar, M. Mahesh Kumar, R. Mallik
and E. V. Sampathkumaran (1999).
La substitution induced linear temperature
dependence of electrical resistivity and Kondo behaviour in the alloys, Ce2-xLaxCoSi3 Solid State
Communications 110, 509.
R. Mallik, E.S. Reddy, P.L. Paulose, S. Majumdar
and E.V. Sampathkumaran (1999).
Transport and magnetic
anomalies due to A-site ionic-size mismatch in La0.5Ca0.5-xBax
MnO3 . J. Phys.: Cond. Matter 11, 4179.
S. Mazumdar, R. Mallik, E.V.
Sampathkumaran, P.L. Paulose and K. V. Gopalakrishnan (1999).
Unexpected modication
of magnetic properties by Y substitution in Eu2PdSi3. Phys. Rev. B 59, 4244.
R. Mallik, E.V. Sampathkumaran, P.L. Paulose, H.
Sugawara and H. Sato (1998).
Magnetic anomalies in
Gd2PdSi3. Pramana - J. Phys. 51, 505.
S. Mazumdar, R. Mallik, E.V. Sampathkumaran and P.L. Paulose (1998).
Residual resistivity
ratio and its relation to the magnetoresistance in LaMn2Ge2
derived alloys.
R. Mallik and E.V. Sampathkumaran (1998)
Magnetic precursor effects, electrical and
magnetoresistance anomalies, and heat capacity behaviour of Gd alloys. Phys. Rev. B 58,
9178.
R. Mallik, E.V. Sampathkumaran, J. A. Alonso and
M. J. Martinez- Lope (1998).
Complex low
temperature transport behavior of RNiO3 - type of compounds. J. Phys.: Cond.
Matter 10, 3969.
R. Mallik, E.V. Sampathkumaran, P.L. Paulose, M.
Strecker, G. Wortmann and Y. Ueda (1998).
Complex magnetism in a
new alloy, Eu2PdSi3, with two crystallographically
inequivalent sites. J. Magn. Magn. Mater. 185, L135 (Letters).
R. Mallik, E.V. Sampathkumaran and P.L. Paulose (1998).
Large low temperature
magnetoresistance and magnetic anomalies in Tb2PdSi3 and
Dy2PdSi3.
R. Mallik, E.V. Sampathkumaran, M. Strecker and
G. Wortmann (1998).
Observation of a minimum in the temperature
dependent electrical resistance above magnetic ordering in a Gd alloy. Europhysics Letters
41, 315.
Sundaresan, P. L. Paulose, R. Mallik
and E. V. Sampathkumaran (1997).
Bandwidth controlled magnetic and electronic
transitions in La0.5Ca0.5-xSrxMnO3
distorted perovskite. Phys. Rev. B 57, 2694.
R. Mallik, E.V. Sampathkumaran and P.L. Paulose (1997).
Large positive magnetoresistance at low
temperatures in a ferromagnetic natural multilayer, LaMn2Ge2 Applied Physics
Letters 71, 2385.
R. Mallik, P.L. Paulose, E.V. Sampathkumaran, S.
Patil and V. Nagarajan (1997).
Coexistence of localized and (induced) itinerant
magnetism and heat-capacity anomalies in Gd1-xYx Ni
alloys
Phys. Rev. B 55, 8369.
R. Mallik, E.V. Sampathkumaran, P.L. Paulose and
V. Nagarajan (1997)
Magnetoresistance in GdNi: Magnetic
polaronic-like effect near the Curie temperature and low-temperature sign
reversal
Phys. Rev. B 55, R8650 (Rapid Communication).
R. Mallik, E.V. Sampathkumaran and P.L. Paulose (1997).
Kondo lattice
behaviour and multiple characteristic temperatures in CeIr2 Ge2 Phys. Rev. B 55,
3627.
R. Mallik, E.V. Sampathkumaran, J. Dumschat and
G. Wortmann (1997).
Magnetic ordering and
spin fluctuation behaviour in compounds of the type Ce2(Pd,Rh)2
R. Mallik and E.V. Sampathkumaran (1996).
Magnetic behaviour of
the alloys (Ce1-x Yx)2PdSi3 J. Magn. Magn. Mater.
164, L13.
E.V. Sampathkumaran, P.L. Paulose and R.
Mallik (1996).
Magnetoresistance
anomalies and multiple magnetic transitions in SmMn2Ge2. Phys. Rev. B 54, R3710
(Rapid Communication).
Comments / Conference proceedings etc.
R. Mallik and Steven P. Gross
Intracellular Transport: How Do Motors Work Together?
Current Biology 19 : R416 (2009)
R. Mallik and Steven P. Gross
Molecular motors as cargo transporters in the cell The
good, the bad and the ugly
Physica A 372, 65 (2006). Proceedings of the Workshop on
"Common Trends in Traffic Systems", IIT-Kanpur, Feb 2006 Download PDF
A.M. Saxena, R. Mallik, J.B. Udgaonkar and G.
Krishnamoorthy, Protein dynamics controls proton transfer from bulk solvent
to interior of proteins: a case study with GFP. 8th Conference on
Methods and Applications of Fluorescence: Spectroscopy, Imaging
and Probes.
A.M. Saxena, R. Mallik, J.B. Udgaonkar
and G. Krishnamoorthy.
Protein Dynamics Controls the Fast Kinetics of
Proton Transfer from Bulk solvent to Interior of Proteins: A case Study with
GFP.
Symposium of the Protein Society: Protein Structure & Function,
Indian Institute of Technology
S.
Majumdar et.
al.,
Physica B 281&282 (2000) 367
S.
Majumdar et.
al.,
Physica B 259&261 (1999) 166
R. Mallik et.
al.,
Physica B 259&261 (1999) 892
S. Majumdar et.
al.,
Physica B 259&261 (1999) 843
R. Mallik et.
al.,
Physica B 230&232 (1997) 169
R. Mallik et.
al.,
Physica B 230&232 (1997) 731
E.V. Sampathkumaran et.
al., Physica B 223&224 (1996) 316
R. Mallik et.
al., Physica
B 223&224 (1996) 382