Tata Institute of Fundamental Research


A Sri Rama Koti

Reader (Adjunct Faculty)

Protein mechanochemistry by single-molecule atomic force microscope

Biochemistry, Biophysics, Molecular Biology

Mechanical forces play a central role in ubiquitous phenomena such as cell-adhesion, tissue organization, and muscle function in multi-cellular organisms. Cell-adhesion is also essential in wound healing, protecting us from microorganisms, and maintaining immunity. Unraveling the molecular mechanism of these important biophysical and mechanochemical processes is an exciting question in modern cell biology. We use state-of-the-aft atomic force microscope (AFM) to probe single molecules and apply stretching forces to study their mechanical response and mechanochemistry.


1. Koti AS, Wiita AP, Dougan L, Uggerud E, Fernandez JM (2008) Single-Molecule force spectroscopy measurements of bond elongation during a bimolecular reaction. Journal of the American Chemical Society 130: 6479
2. Koti AS, Wiita AP, Huang HH, Fernandez JM (2008) A Single-Molecule Assay to Directly Identify Solvent Accessible Disulfide Bonds and Probe Their Effect on Protein Folding. Journal of the American Chemical Society 130: 436
3. Koti AS, Brujic J, Huang HH, Wiita AP, Li L, Lu H, Walther K, Carrion-Vazquez M, Li H, Fernandez JM (2007) Contour length and refolding rate of a small protein controlled by engineered disulfide bonds. Biophysics Journal 92: 225
4. Wiita AP, Koti AS, Huang HH, Fernandez JM (2006) Force-dependent chemical kinetics of disulfide bond reduction observed with single molecule techniques. Proceedings of National Academy of Sciences USA 103: 7222
5. Koti AS, Li L, Badilla CL, Fernandez JM (2005) Ligand binding modulates the mechanical stability of dihydrofolate reductase (DHFR). Biophysical Journal 89: 3337


  • Room FTNMR2 (Department of Chemical Sciences)
  • Tel: +91 22 2278 2790
  • koti @ tifr.res.in
  • Webpage