Title: Fibrils, membranes, crystals, sediments: solid-state NMR of large proteins

Abstract :

Solid-state NMR is an increasingly powerful tool to characterize challenging proteins. Notably, it can analyze an astonishing variety of states, as proteins inserted in membranes, crystals and simple sediments. Combined with other biophysical approaches, solid-state NMR thus gives unique insight into protein structure, and ultimately function. We will illustrate this with some recent examples from our laboratories, including the DnaB helicase from Helicobacter pilori, the BmrA ABC transporter, as well as the yeast prion fibrils Ure2p and Sup35p, for which we will show results related to sample preparation, sequential assignments and structural aspects.