Title : Towards Nonlinear Label-Free Imaging of Monoamines in Live Vertebrates

Abstract :

Monoamines are an important class of neurotransmitters that act to transmit signals from one neuron to the next.  They play a significant role in the processing of emotion, reward, sleep-wake cycle and many different behaviors and imbalance of these neurotransmitters believed to be the cause of many neurodegenerative diseases like Parkinson’s, Alzheimer’s, Schizophrenia etc.  Direct imaging of monoamines by single photon excitation has proven to be difficult because their excitation resides in the ultra violet regions.  While some success has been obtained in imaging monoamine neurotransmitters by multi-photon excitation in cells and tissue, the holy grail will be to image it in vivo in live animals.  The present talk will describe our first attempts to use multi-photon label-free imaging to observe monoamine neurotransmitters in live vertebrates (zebra fish) and the corresponding instrumentation.